The scientist will be responsible to run in vitro primary cell culture in 96-well plate format, perform serial dilutions of drug molecules, perform ELISA for cytokine analysis, and perform FACS flow cytometry analysis to look at cell populations changes and cell phenotype changes. Candidates with previous mRNA LNP production and characterization, and LNP antibody conjugation are preferred.
1. In vitro primary cell culture (human PBMC, Cyno PBMC and mouse splenocyte)
2. Serial dilutions of drug molecules and application of treatment using multichannel pipette
3. Live/Dead and antibody staining of cells and FACS flow cytometry run on BD FACS Canto II / Fortessa / LSR II and data analysis using FlowJo software
4. Good to have: mRNA LNP production and characterization
5. Good to have: LNP antibody conjugation
3-5 year experience level
Masters preferred
About US Tech Solutions:
US Tech Solutions is a global staff augmentation firm providing a wide range of talent on-demand and total workforce solutions. To know more about US Tech Solutions, please visit www.ustechsolutions.com.
US Tech Solutions is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran.